Motif‐directed redesign of enzyme specificity
نویسندگان
چکیده
منابع مشابه
Motif-directed redesign of enzyme specificity
Computational protein design relies on several approximations, including the use of fixed backbones and rotamers, to reduce protein design to a computationally tractable problem. However, allowing backbone and off-rotamer flexibility leads to more accurate designs and greater conformational diversity. Exhaustive sampling of this additional conformational space is challenging, and often impossib...
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Site-directed mutagenesis is still a very efficient strategy to elaborate improved enzymes. Recently, advances have been made in developing rational strategies aimed at reshaping enzyme specificities and mechanisms, and at engineering biocatalysts through molecular assembling. These knowledge-based studies greatly benefit from the most recent computational analyses of enzyme structures and func...
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ژورنال
عنوان ژورنال: Protein Science
سال: 2014
ISSN: 0961-8368,1469-896X
DOI: 10.1002/pro.2417